This is the cover page image published in Developmental Cell, August 14 2012. The Image was taken at Center for Electron Microscopy and Nanofabrication, Portland State University by instrument manager Greg Baty to support the post doctorial research work of Katie Kindt at OHSU (Teresa Nicolson lab Oregon Hearing Research Center). The research was funded by NIH and HHMI grant. The image is of a Zebra fish neuromast taken near the ear. Katie Kindt false colored the SEM image taken by Greg Baty Katie’s main interest in taking the SEM image was to examine the stereocilia and correlate the result with confocal studies that where performed while the zebra fish was alive. Katie and Gabe Finch at OHSU had a difficult time preparing the fish for SEM, due to the variability in a rapidly growing fish that is three days old. It was necessary to perform some digestion to expose the cilia for fixation. This was a very difficult imaging job for Greg since CEMN does not have a sputter coater with a tilting orbital stage and our Sirion is a high vacuum only instrument. The length and geometry of the cilia combined with charging due to poor coating tends to cause the celia to move in the electron beam. It took an interdisciplinary team effort to produce an image of this quality on a high vacuum XL30 Sirion. K. S. Kindt, G. Finch, and T. Nicolson, "Kinocilia Mediate Mechanosensitivity in Developing Zebrafish Hair Cells", Developmental Cell, Vol 23, (2), pgs 329-341 (2012). Katie Kindt [email protected] Greg Baty [email protected] Greg Baty [email protected] Courtesy of Greg Baty Image Details Instrument used: Sirion Family Magnification: 9379x Horizontal Field Width: 13.2 Vacuum: 7e-6 mbar Voltage: 2 Spot: 3 Working Distance: 4.1 Detector: TLD
Red Zebrafish
Dr Don Love and his Auckland University team use zebrafish to study heritable human diseases such as Duchenne muscular dystrophy, Huntington's disease, and inflammatory bowel disease. Like any research involving animals, their work is subject to strict ethical scrutiny.
A world of detail in an embryo less than a day old.
Quick post tonight, because I am up to my elbows in icing trying to whip out several hundred cookies for a holiday party this weekend. Tonig...
With their beauty, energy, and ease of care, it’s no wonder Zebra Danios are very popular in the fish-keeping world. Also know as Zebrafish or by their scientific name danio rerio, they're great for both beginner and experienced fish-keepers alike! But understanding the essentials of Zebra Danio care is of…
Today we have the 2nd in a series of articles by Jan Botthof, a PhD Student at the Cambridge University Department of Haematology and the world renowned Wellcome Trust Sanger Institute. Following his first article "Zebrafish: the rising star of animal models", Jan discusses here how Zebrafish used in scientific research are housed, cared for…
Discover How Long Zebra danio or zebrafish Lives
Fish learned to avoid a predator robot after just a minute of training, retaining this memory for over an hour.
Fengzhu Xiong, Sean Megason (Harvard Medical School)See also http://www.olympusbioscapes.com/gallery/2011/index.htmlThis movie provides a single-cell resolut...
Read more about two new drugs that were seen to reverse peripheral neuropathy in zebrafish and may be a treatment for conditions including MS.
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Read more about how researchers at the University of Edinburgh will use zebrafish to study the role of CD46 protein in MS inflammation and its process.
The zebra danio is a freshwater species of the Cyprinidae family. Also called zebrafish, zebra danios are popular due to their beautiful...
Scientists at Berekely Lab have developed a new technique that images every pigment cell of a whole zebrafish in 3D.
Recent transcriptome annotation using deep sequencing approaches have annotated a large number of long non-coding RNAs in zebrafish, a popular model organism for human diseases. These studies characterized lncRNAs in critical developmental stages as well as adult tissues. Each of the studies has uncovered a distinct set of lncRNAs, with minor overlaps. The availability of
Julien Resseguier - Network of macrophages (white blood cells) of an adult zebrafish intestine
Abstract. Atlases provide a framework for spatially mapping information from diverse sources into a common reference space. Specifically, brain atlases all
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