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Observing the structural dynamics of biomolecules is vital to deepening our understanding of biomolecular functions. High-speed (HS) atomic force microscopy (AFM) is a powerful method to measure biomolecular behavior at near physiological conditions. In the AFM, measured image profiles on a molecular surface are distorted by the tip shape through the interactions between the tip and molecule. Once the tip shape is known, AFM images can be approximately deconvolved to reconstruct the surface geometry of the sample molecule. Thus, knowing the correct tip shape is an important issue in the AFM image analysis. The blind tip reconstruction (BTR) method developed by Villarrubia (J Res Natl Inst Stand Technol 102:425, 1997) is an algorithm that estimates tip shape only from AFM images using mathematical morphology operators. While the BTR works perfectly for noise-free AFM images, the algorithm is susceptible to noise. To overcome this issue, we here propose an alternative BTR method, called end-to-end differentiable BTR, based on a modern machine learning approach. In the method, we introduce a loss function including a regularization term to prevent overfitting to noise, and the tip shape is optimized with automatic differentiation and backpropagations developed in deep learning frameworks. Using noisy pseudo-AFM images of myosin V motor domain as test cases, we show that our end-to-end differentiable BTR is robust against noise in AFM images. The method can also detect a double-tip shape and deconvolve doubled molecular images. Finally, application to real HS-AFM data of myosin V walking on an actin filament shows that the method can reconstruct the accurate surface geometry of actomyosin consistent with the structural model. Our method serves as a general post-processing for reconstructing hidden molecular surfaces from any AFM images. Codes are available at https://github.com/matsunagalab/differentiable_BTR .
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This video is all about supports. Which types are available, how they are displayed, what they look like, and much more. In simple terms, supports are the connections between a structure and a fixed point. Depending on the support type, they prevent certain displacements or rotations of the structure. We show you the most common types in structural engineering, which movements they allow (the so-called degrees of freedom) and the respective support forces that can occur. Have fun! Infotainment videos are primarily used for gathering information, but also for entertainment. Animated videos help to combine the knowledge and theory in the study with practical applications from the construction industry. Learn about our programs by using our e-learning videos: ► Tutorials for Students: https://www.youtube.com/playlist?list=PLx7dtCtXeyHUF8P9CxdOvFnyiq7Qq4N1Y ► RFEM 5 for Beginners: https://www.youtube.com/playlist?list=PL8ofwN6MZdI-qqZzPjs5V56mVGL0c1-CV ► Modeling with RFEM 5: https://www.youtube.com/playlist?list=PLx7dtCtXeyHX-eORa5XIvjeWG57_z4PG1 Did you find this video helpful? ► Then we look forward to your comment on this video and a Like. Dlubal Software supports students and educational institutions: ► Free Software for Students https://www.dlubal.com/en-US/education/students/request-or-renew-free-student-license ► Structural Analysis Software for Educational Institutions: https://www.dlubal.com/en-US/education/schools/free-structural-analysis-software-for-students Follow us and stay updated on our current news: ► Subscribe to Dlubal YouTube Channel: https://www.youtube.com/user/DlubalEN?sub_confirmation=1 ► Instagram: https://www.instagram.com/dlubal_software/ ► Are you also on Facebook? Follow us here: https://www.facebook.com/dlubal.software.english Keywords: #DlubalInfotainment #Support #RollerSupport #PinnedSupport #FixedSupport #RestrainedSupport #NodalSupport #ELearning #Entertainment #ConstructionIndustry #CivilEngineering #CivilEngineer #Studies #StructuralEngineering #StructuralEngineer #CivilEngineeringStudies #StructuralAnalysis
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In diesem Video wird der Unterschied zwischen Stablasten und Linienlasten anhand von zwei Modellen erläutert. Das Video erklärt dabei auf welche Elemente Stablasten und Linienlasten angesetzt werden.
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In diesem Video werden die beiden Möglichkeiten absoluter Versatz und relativer Versatz zur Erzeugung von Stabexzentrizitäten gezeigt. Es wird erläutert welchen Einfluss eine Exzentrizität auf die resultierenden Schnittgrößen haben kann. Innerhalb der Videoreihe "Tipps & Tricks" werden nützliche Funktionen und Werkzeuge vorgestellt, die helfen, die Produktivität zu steigern und bereits erworbene Kenntnisse zu erweitern. Zusatzinformationen zu diesem Tutorial sowie Modell zum Herunterladen: ► Dlubal-Website: https://www.dlubal.com/de/support-und-schulungen/schulungen/videos/002027 Hat Dir das Video gefallen oder weitergeholfen? ► Dann freuen wir uns über Ihren Kommentar zu diesem Video und einen Like. Unser Support ist kostenlos. Hast Du eine Frage? ► Häufig gestellte Fragen (FAQs): https://www.dlubal.com/de/support-und-schulungen/support/faq ► Individuelle Frage stellen: https://www.dlubal.com/de/support-und-schulungen/support/individuelle-frage-stellen Folge uns und bleibe auf dem Laufenden: ► Dlubal-YouTube-Kanal abonnieren: https://www.youtube.com/user/Dlubal?sub_confirmation=1 ► Instagram: https://www.instagram.com/dlubal_software/ ► Bist Du auch bei Facebook? Folge uns hier: https://www.facebook.com/dlubal.software.deutsch Schlüsselwörter: #TippsUndTricks #Exzentrizität #ExzentrischerStab #ExzentrizitätAmStab #Stabexzentrizität #Stabversatz #AußermittigeStabachse #MomentAusExzentrizität #T&T #ELearning #Tutorial #Kurzvideo
What exactly does an architect do? Structural engineer? Steel worker? Designer? Structures is where it all takes shape. Kids learn about the various components and jobs within the building industry as they role play and imagine themselves hard at work. From drawing blue prints to exploring structural stability requirements, and from connecting with blocks and exploring materials,
The campus of the École Polytechnique Fédérale of Lausanne is on Lake Leman’s shores, with views of the Alps. The...
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The new landscape for Dwight Englewood Middle School creates an engaging outdoor learning environment and sheltered commons.
In diesem Video wird gezeigt, wie Flächenexzentrizitäten modelliert werden können. Es wird erläutert welchen Einfluss eine Exzentrizität auf die resultierenden Schnittgrößen haben kann. Innerhalb der Videoreihe "Tipps & Tricks" werden nützliche Funktionen und Werkzeuge vorgestellt, die helfen, die Produktivität zu steigern und bereits erworbene Kenntnisse zu erweitern. Zusatzinformationen zu diesem Tutorial sowie Modell zum Herunterladen: ► Dlubal-Website: https://www.dlubal.com/de/support-und-schulungen/schulungen/videos/002353 Hat Dir das Video gefallen oder weitergeholfen? ► Dann freuen wir uns über Ihren Kommentar zu diesem Video und einen Like. Unser Support ist kostenlos. Hast Du eine Frage? ► Häufig gestellte Fragen (FAQs): https://www.dlubal.com/de/support-und-schulungen/support/faq ► Individuelle Frage stellen: https://www.dlubal.com/de/support-und-schulungen/support/individuelle-frage-stellen Folge uns und bleibe auf dem Laufenden: ► Dlubal-YouTube-Kanal abonnieren: https://www.youtube.com/user/Dlubal?sub_confirmation=1 ► Instagram: https://www.instagram.com/dlubal_software/ ► Bist Du auch bei Facebook? Folge uns hier: https://www.facebook.com/dlubal.software.deutsch Schlüsselwörter: #TippsUndTricks #Exzentrizität #Flächenexzentrizität #MomentAusExzentrizität #Versatz #T&T #ELearning #Tutorial #Kurzvideo
Engineering Artificial Intelligence ( A.I.) for Dynamic Communication is being sustainable Development in Field of Digital Platforms in Reality.
Classification of columns as short or long ((4 Examples)) 4.1.. The frame shown in the figure below is composed of members with rectangular cross-sections. All members are constructed of the same strength concrete (E is the same for both beams and columns). Considering bending in the plane of the frame only, classify column EF as short if the frame is braced. All girders are 300 x 600 mm.
Temper your jealousy, teachers. One can always dream.
Image 5 of 20 from gallery of New Shoots Children’s Centre / Collingridge And Smith Architects (CASA). Photograph by Amanda Aitken