The purpose of real-time PCR is to perform efficient amplification of a target sequence and quantify the PCR products in “real time” by employing the use of a fluorescent reporter. Fluorescent rep…
Over the past three decades, luminescence spectroscopy has transcended its origins as a curiosity in the physical laboratory to become a widely used and respected staple of the analytical chemist's instrumentation arsenal. Fluorescence, chemiluminescence, and phosphorescence spectroscopies are now routinely applied to such real analytical problems as the quantitaion, qualitative identification, and structural characterization of organic and inorganic compounds and even of cellular structures. And the list of recent advances in analytical applications of luminescence spectroscopy keeps growing. The earlier volumes of Molecular Luminescence Spectroscopy provided professional chemists with a detailed, exhaustive, and up-to-date look at the applications of fluorescence, phosphorescence, and chemiluminescence spectra to the analysis of organic and inorganic compounds. Presenting topics never available in any analytical text, such as excited state optical activity and bioinorganic luminescence spectroscopy, the volumes represented a significant advance in the chemical literature. Part 3 continues the book's always current and practical examination of the field's newest innovative turns. In a clear, systematic format, Part 3 discusses such widespread or ascendant laboratory techniques as: photochemically generated fluorophores fluorescent probes luminescence from bile salt aggregates hole-burning spectroscopy laser-excited microspectro-fluorometry near-infrared luminescence spectroscopy Other topics such as the fluorescence and phosphorescence of pharmaceuticals and natural products have never been reviewed as exhaustively before. The chapters on fluorescence detection in chromatography and luminescence immunoassay are the most up-to-date treatments available on these subjects. Invaluable to analytical chemists, instructors, and students, Molecular Luminescence Spectroscopy, Part 3 offers expert guidance on the practical specifics of this multi-faceted technique as well as its farreaching analytical possibilities.
The purpose of real-time PCR is to perform efficient amplification of a target sequence and quantify the PCR products in “real time” by employing the use of a fluorescent reporter. Fluorescent rep…
On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become \"capable proactive researchers.\" Features: - Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques - Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations - Discusses fluorophores, and solvent effects on protein structure - Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature - Includes carefully selected primary source research literature and articles from current vendor literature - Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized - Provides an alphabetized list of common reagents for rapid reference - Offers an extensive index of concepts and terms - Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches
