The sitter could have been a number of Lady Ratcliffes. Sir Robert Ratcliffe, Ist Earl of Sussex (1483–1542), had three wives, of which the third, Mary, is most possible. Art historian K. T. Parker tentatively favoured his son Henry's wife Lady Elizabeth Howard (d. c. 1536), daughter of Thomas Howard, 2nd Duke of Norfolk, and his second wife, Elizabeth Tilney, as the most plausible sitter, since Holbein drew other members of the Howard family. That would make this woman Anne Boleyn's Aunt. Anne's maternal grandfather, the 2nd duke, was twice married, first to Agnes Tilney. Anne's mother, Elizabeth Howard, was born circa 1480. The Duke took for his second wife, after Agnes's death, Elizabeth Tilney, and this Elizabeth was her daughter, born sometime around 1513. Reference * K. T. Parker, The Drawings of Hans Holbein at Windsor Castle, Oxford: Phaidon, 1945, OCLC 822974, p. 41.